Resistance to Drug (Resistanceto Drug) refers to the tolerance of microorganisms, parasites and tumor cells to the action of chemotherapeutic drugs. Once drug resistance occurs, the chemotherapy effect of drugs is significantly reduced. Therefore, the treatment of rice seedling disease with prochloraz should prevent drug resistance.
Rice seedling disease is an important disease on rice. The benzoxazole drug represented by carbendazim was the main agent for the prevention and treatment of this disease. With the resistance of the pathogen to the carbendazim, the single use The drug control effect is reduced. Prochloraz is an imidazole fungicide. In recent years, it has achieved good control effect against rice seedling disease, but at present some local areas have also experienced a decline in control effect.
In this regard, the researchers of the College of Agronomy and Biotechnology of China Agricultural University and the Institute of Pesticide Testing of the Ministry of Agriculture used mycelial growth rate and microscopic observation to study the growth of sensitive strains of M. meliloti and resistant mutant strains. Effects of development and mycelial growth morphology. The results showed that: 0.5 mg / liter of prochloraz solution can completely inhibit the mycelial growth of sensitive strains, can not inhibit the mycelial growth of resistant strains with different resistance levels; 20 mg / liter of prochloraz solution can completely inhibit the parents The spore germination of sensitive strains inhibited the spore germination and germ tube elongation of different resistant mutants by 84.1%-89% and 58.5%-65.8%, respectively, indicating that the resistant mutant strains grew in mycelium, spore germination, The sensitivity to the agent at the stage of elongation of the germ tube is reduced to a different extent than that of the parental sensitive strain. After treating the hyphae with 10 mg/L of prochloraz solution for 12 hours, the hyphae of the parental sensitive strain expanded and deformed, and the hyphal top of the resistant mutant expanded, but there were many small branches on the hyphae, indicating that the agent was antagonistic. The inhibitory effect of the secondary mutant hyphae of the mutant was not obvious.
The above test results indicate that the rice seedling pathogen has potential drug resistance risk to prochloraz , and it is recommended to rotate the drug with a different mechanism of action such as dithiocyanomethane or trichloroisocyanuric acid to delay Or avoid the emergence of drug resistance.
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